FIG. 8.

Sulforaphane increases the cellular Nrf2 response. (A) LLC-PK1 cells were stained for Nrf2-positive nuclei. Shown are representative pictures of control cells and aldosterone- (10 and 100 nM) and sulforaphane (2 μM)-treated cells. Pictures were taken by a TCS SP5 laser scanning confocal microscope. Quantifications were done by measuring gray values of ∼100 cells per treatment after 4 h (B) and 24 h (C) with ImageJ. (D) Nrf2 nuclear abundance normalized to the respective control after 4 and 24 h of aldosterone treatment. (B–D) Shown are mean values±SEM, *p≤0.05 versus the control group, °p≤0.05 versus 10 nM aldosterone alone, ^Δp≤0.05 versus 100 nM aldosterone alone, tested by ANOVA with subsequent post-hoc comparisons by Scheffé. See Supplementary Figure S3.