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. 2014 Oct 1;3(5):206–216. doi: 10.1089/biores.2014.0028

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Copyright 2014, Mary Ann Liebert, Inc.

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FIG. 3. — Morphological architecture of H9 hIPSCs cultured on murine embryonic fibroblasts (MEFs). (A) Semi-thin section of H9 hIPSCs. This panel illustrates the general architecture of a colony The IPSCs are organized in colonies with microvilli at the upper side (A1). These cells have a high nucleo-cytoplasmic ratio and present a variable number of nucleoli (A2, A3, A4, A5). Some cells are in mitosis (A6). Optical microscopy, original magnification ×400. (B) The cells at the upper side of the colony present several microvilli of nonintestinal type (arrow). TEM, original magnification ×15,000. (C) The cells are closely related according to the presence of zonula adherens and macula adherens (arrow) with their intermediate filaments on each cytoplasmic side of the membranes. TEM, original magnification ×15,000. (D) The cells inside the colonies had a high nucleo-cytoplasmic ratio and very significant intercellular spaces (arrow). TEM, original magnification ×5000. (E) Presence of ducts inside the colony (arrow). These ducts are formed by apical differentiation of several epithelial cells. Inside these ducts are fractions of cell elements. TEM, original magnification ×6000.