Fig. 6.

Identification of TD-modified proteins with specific antibodies. (A) Immunoprecipitation of TD-modified proteins by 1B7 mAb-beads. (B) Precipitation of biotinylated proteins by avidin-beads. The cells were exposed to 100 µM of synthetic TD or TD–biotin and then lysed. The pull-down assay was performed as described in Materials and methods. The precipitated proteins were stained with SYPRO Ruby.