FIG. 6.
Captopril attenuates HBOC-induced NAD(P)H oxidase subunit overexpression and activity. (A–C) Western blot analysis of NAD(P)H oxidase subunits expressions (p47phox, p67phox, gp91phox, Nox1, and Nox4) in coronary arteries. The blot is a representative of blots obtained from five independent experiments. (D) NAD(P)H oxidase activity in HUVECs. (E, F) Representative of immunohistochemical staining and quantification analysis for gp91phox-positive protein in coronary arteries. Scale bar: 50 μm. Values are presented as mean±SD. *p<0.05, **p<0.01, and ***p<0.001 versus the 0.1%HBOC group; #p<0.05 versus the 3%HBOC group. ACEI in the figure indicates ACE inhibitor captopril. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars