Fig. 2.

(A) Photographs of 2-dimensional electrophoresis protein separation of pooled sputum (n = 5) obtained from uncontrolled asthmatics with neutrophilic inflammation (>70%). Protein spots are dentified by matrix-assisted laser desorption/ionizatione time of flight/time of flight. Enlargements of spots 2 is S100 calcium binding protein A9 (S100A9). (B) Comparison of S100A9 levels in sputum between the neutrophilic (neu) and eosinophilic (eos) subtypes of severe uncontrolled asthma (UA), partially controlled asthma (PA), and controlled asthma (CA) Using enzyme-linked immunosorbent assay. Data are presented as median (interquartile range). ^*p < 0.01 vs. neutrophilic subtypes of severe uncontrolled asthma. Adapted from Lee TH, et al. Ann Allergy Asthma Immunol 2013;111:268-275.e1, with permission of Elsevier B. V. [62].