Fig. 3.

Representative sections stained for axonal neurofilament using SMI312 immunohistochemistry (A–E). Quantitative differences of gray levels in the ipsilateral (Ipsil) and contralateral (Contral) hemisphere were presented as a % of Contral (panel F), there were significant reductions in gray level ipsilateral to the hypoxia–ischemia (HI), in the parietal cortex at 3 h and 1 d. Cerebral peduncle (arrows) showed decreased gray levels at acute and chronic time points (1 d to 4 w). Also shown is a representative section demonstrating diminished myelin basic protein (MBP) staining within the cerebral peduncle at 4 w following HI (G). Data are presented as mean ± SD. *P < 0.05; ipsilateral different from contralateral; ^†P < 0.05, cortex or pons different from cerebral peduncle within the time point; ^‡P < 0.05, different from corresponding region at 1 d; ^ψP < 0.05 different from corresponding region at 3 h (Two Way repeated ANOVA, Holm–Sidak multiple comparisons).