Fig. 4.

Neuronal cell death detected with fluorojade staining at various times after transient unilateral cerebral hypoxia–ischemia. Shown are representative sections from parietal cortex of a control rat with sham surgery (A) and from cortex at 2 h, 2 d and 4 w after hypoxia–ischemia (B−D, respectively). The fluorojade positive cells are readily seen within the higher magnification insets for each micrograph (scale bars = 50 µm). Positive stained cells in the cerebral peduncle region at 2 d post insult are observed ipsilateral (F) but not contralaterally (E). The quantitative differences in numbers of stained cells per field of view ipsilaterally compared to that contralaterally after hypoxia–ischemia was significant only for parietal cortex (G). Data are presented as mean ± SD. *P < 0.05; ipsilateral different from contralateral; ^†P < 0.05, cortex or pons different from cerebral peduncle within the time point; ^‡P < 0.05, different from corresponding region at 3 h and 1 d (Two Way repeated ANOVA, Holm–Sidak multiple comparisons).