Fig. 3.

Ethanol-induced FoxO3a-mediated transcription of autophagy related and FoxO3a target genes were inhibited in FXR KO mouse livers. Age matched WT and FXR KO mice were treated with 4.5 g/kg ethanol or water by gavage for 16 h. Hepatic mRNA was isolated and qRT-PCR was performed for autophagy related genes, Atg5, Becn-1, and MAP1LC3B (A) and FoxO3a target genes, MnSOD, p21, and FoxO3a (B). qRT-PCR was also performed for FXR target gene, Shp (C). The gene expression levels were normalized to β-actin and shown as fold increase over wild type mice (n=4–7). *p<0.05. One-way ANOVA analysis. WT control vs FXR KO control, WT ethanol vs FXO KO ethanol.