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. 2014 Sep 30;45(6):2232–2240. doi: 10.3892/ijo.2014.2688

Figure 3.

Figure 3

Nuclear CXCR5 expression in lung cancer (LuCa) tissues. (A) Representative figures of non-neoplastic (n=8), squamous cell carcinoma (SCC) (n=22), and adenocarcinoma (AC) (n=52) lung tissues stained with isotype control or anti-CXCR5 antibodies. Brown [3,3′-diaminobenzidine (DAB)] color show CXCR5 staining. An Aperio ScanScope CS system with a 40× objective captured digital images of each slide. Stained cells with negative and positive nuclei were counted and categorized according to stain intensity 0 (Blue), 1+(yellow), 2+(orange) and 3+(Red). (B) The nuclear intensity of CXCR5, in non-neoplastic (n=8), SCC (n=22), and AC (n=52) tissues, quantified using a nuclear algorithm of image analysis Aperio ImageScope v.6.25 software. *Significant differences (p<0.001) between groups with LuCa and control. (C–F) Nuclear intensities of CXCR5 in different tumor stages and in tumors with nodal involvement in SCC and AC cases, respectively.