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. 2014 Sep 26;45(6):2365–2372. doi: 10.3892/ijo.2014.2677

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Copyright © 2014, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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The expression profiles of stemness-genes in ALDH1^pos and ALDH1^neg cell populations in FA-HNSCC. The Aldefluor assay was used to isolate ALDH1^pos and ALDH1^neg cells in VU1365 cell line and used for RNA isolation and reverse transcription-polymerase chain reaction (RT-PCR) array analysis. The human pluripotent stem cell assessment primer pairs were used to amplify the mRNA transcripts of 14 genes that are frequently used as molecular markers of undifferentiated embryonic stem (ES) cells (Lane 2, DPPA5/ESG1; Lane 3, Nanog; Lane 4, Oct-3/4; Lane 5, SOX2), ectodermal lineage-committed stem cells (Lane 5, SOX2; Lane 6, Nestin; Lane 7, Otx2; Lane 8, TP63), endodermal lineage-committed stem cells (Lane 9, AFB; Lane 10, GATA-4; Lane 11, SOX17; Lane 12, HNF-3β), mesodermal lineage-committed germ cells (Lane 13, Brachury) and germs cells (Lane 14, Stella). Lane 15, GAPDH-loading control.