Table 1.
Summary of primary antibodies
| Antigen | Immunogen | Manufacturing details (including animal raised in) | Working dilution |
|---|---|---|---|
| SMI32 | Rat hypothalamus homogenate | Covance Research Products Inc. (Emeryville, CA, USA), mouse monoclonal, cat. no. SMI32R | 1 : 1000 |
| ChAT | Human placental enzyme ChAT | Millipore Corporation (Temecula, CA, USA), rabbit polyclonal, cat. no. AB143 | 1 : 1000 |
| ChAT | 22 amino-acid peptide from porcine ChAT coupled to KLH | Advanced Targeting Systems (San Diego, CA, USA), rabbit polyclonal, cat. no. AB-N34ap | 1 : 1000 |
| p75NTR | Clon ME20.4, WM245 melanoma cells | Advanced Targeting Systems, mouse monoclonal, cat. no. AB-N07 | 1 : 500 |
| GAD67 | Recombinant fragment (region within AA 5 and 176 of GAD67) | Abcam plc (Cambridge, UK), rabbit polyclonal, cat. no. ab97739 | 1 : 100 |
| Parvalbumin | Frog muscle parvalbumin (clone PARV-19) | Sigma–Aldrich (Dorset, UK), mouse monoclonal, cat. no. P3088 | 1 : 6000 |
| Green fluorescent protein (GFP) | GFP-tagged fusion protein (clone GFP-20) | Sigma–Aldrich (Dorset, UK), mouse monoclonal, cat. no. G6539 | 1 : 6000 |
| Tetramethyl-rhodamine dextran | Tetramethyl-rhodamine | Molecular Probes Inc. (Eugene, OR, USA), rabbit polyclonal, IgG fraction, cat. no. A6397 | 1 : 6000 |
| Fluorescein dextran | Fluorescein/Oregon green | Molecular Probes Inc., rabbit polyclonal, IgG fraction, cat. no. A889 | 1 : 6000 |
| Cascade blue | Alexa Fluor 405/cascade blue | Molecular Probes Inc., rabbit polyclonal IgG fraction, cat. no. A5760 | 1 : 6000 |