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. 2014 May 13;16(5):698–704. doi: 10.4103/1008-682X.129209

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Copyright: © Asian Journal of Andrology

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Relative quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis of germ cell specific genes. Relative messenger ribonucleic acid expression levels were analyzed by relative quantitative RT-PCR. Each group contains five mice. One side of the testis (left) was injected with saline, HEK293 cells and HUC-MSCs separately. The other side (right) was as control without injection. After 3 weeks,10 of germ cell associated genes were detected. β-actin was used as an internal gene control. The value of each mouse was calculated by the testis which injected compared to the control side. Five mice were calculated for each gene. Compared to the saline-treated group and HEK293 cells group, the expression of germ-cell-specific-genes in UC-MSCs-injected group was higher than the control sides. Results are expressed as the mean ± s.d. for experiments * P ≤ 0.05.