Figure 4.

Western blot analysis of germ cell specific genes. (a) Detection of miwi, (b) vasa, (c) Scp3 protein expression by western blot analysis. The expression of β-Actin was used as a control gene. The statistics method is similar to messenger ribonucleic acid expression. In each group five mice were selected. Expression of miwi, vasa, Scp3 was detected at a higher level in injected testis than control side on week three after injecting HUC-MSCs compared to another group. Results are expressed as the mean ± standard deviation for experiments * P ≤ 0.05.