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ROS inhibition abolishes the LPA-induced increase in FN-integrin binding. (A) Incubation of VSMC with LPA (2 μM) for 2 h in the presence of tempol (250 μM, n = 36) results in a 47% decrease in FN-integrin adhesion compared with LPA alone (n = 35). (B) Compared with LPA alone (n = 40), 2 h of LPA (2 μM) treatment, in the presence of apocynin (300 μM, n = 39), reduced FN-integrin adhesion by 59%. Data are means ± s.e.m. *P < 0.05 vs. LPA alone.
