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NPHP4 at the transition zone is static. Wild-type (WT) gametes were mixed with NPHP4–HAN (A) or NPHP4–HAC (B) gametes to initiate the mating reaction. At the indicated times after mixing, nucleoflagellar apparatuses of the resulting quadriflagellated zygotes were prepared, fixed and stained with anti-acetylated tubulin, anti-HA and DAPI. The right panels are enlargements of the transition zones shown in the merged images. Scale bars: 1 µm.
