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. Author manuscript; available in PMC: 2014 Oct 31.
Published in final edited form as: Biomaterials. 2013 Feb 4;34(13):3256–3269. doi: 10.1016/j.biomaterials.2013.01.028

Table 1.

Statistical analysis of differences in mass spectrometry from de-cellularized aged and emphysematous and fibrotic injured mouse lungs.

Gene Protein name vs. RLL
vs. Elast
vs. Oldlg
IPI
Old lung Elast Old elast Bleo Old elast Old elast
Cytoskeletal
Actn4 Alpha-actinin-4 0.200 1.000 0.470 0.048 0.643 0.467 IPI00118899.1
Cbr2 Carbonyl reductase [NADPH] 2 0.200 0.821 0.320 0.905 0.952 0.043 IPI00128642.1
Flna Isoform 1 of Filamin-A 0.086 0.107 0.002 0.310 0.024 0.005 IPI00131138.10
Plec Plectin isoform 1b2alpha 0.200 0.833 0.106 0.750 0.024 0.005 IPI00229509.3
Spna2 Isoform 2 of Spectrin alpha chain, brain 0.810 0.833 0.011 0.036 0.012 0.014 IPI00753793.2
Tuba1a Tubulin alpha-1A chain 0.005 0.119 0.087 0.012 0.714 0.010 IPI00110753.1
Tuba1b Tubulin alpha-1B chain 1.000 1.000 0.061 0.012 0.155 0.133 IPI00117348.4
Tubb2b Tubulin beta-2B chain 0.910 0.571 0.022 0.119 0.071 0.010 IPI00109061.1
Vcl Vinculin 0.952 0.357 0.106 0.214 1.000 0.010 IPI00405227.3
Vim Vimentin 0.048 0.714 0.158 0.381 0.012 0.005 IPI00227299.6
Extracellular matrix proteins
Col1a2 Collagen alpha-2(I) chain 0.281 0.048 0.002 1.000 0.012 0.067 IPI00222188.4
Col4a2 Collagen alpha-2(IV) chain 0.019 0.012 0.013 0.012 0.762 0.524 IPI00338452.3
Col6a1 Collagen alpha-1(VI) chain 0.005 0.274 0.013 0.024 0.036 0.381 IPI00339885.2
Col6a2 Collagen alpha-2(VI) chain 0.938 0.155 0.485 0.036 0.393 0.829 IPI00621027.2
Col6a3 collagen alpha-3(VI) chain 0.133 0.024 0.004 0.702 0.238 0.019 IPI00830749.3
Eln Elastin 0.619 0.548 0.632 0.024 0.738 0.381 IPI00134505.1
Emilin1 EMILIN-1 0.057 0.012 0.002 0.012 1.000 0.033 IPI00115516.1
Fbln5 Fibulin-5 0.033 0.083 0.028 0.929 1.000 1.000 IPI00323035.3
Fbn1_1 Fibrillin-1 1.000 0.083 1.000 0.012 0.083 1.000 IPI00122438.1
Fga Fibrinogen, alpha polypeptide isoform 2 0.467 0.750 0.455 0.012 0.333 1.000 IPI00115522.3
Fgb Fibrinogen beta chain 0.957 0.810 0.182 0.012 0.333 0.033 IPI00279079.1
Fgg Putative uncharacterized protein 0.043 0.202 0.015 0.012 0.333 1.000 IPI00122312.2
Fn1_1 Fibronectin 0.871 0.024 0.303 0.012 0.202 0.400 IPI00113539.2
Hspg2_1 Basement membrane-specific heparan sulfate proteoglycan core protein 0.005 0.333 0.275 0.262 1.000 0.043 IPI00113824.1
Hspg2_2 Basement membrane-specific heparan sulfate proteoglycan core protein 0.033 1.000 0.028 0.881 0.012 1.000 IPI00515360.8
Lama4 Laminin subunit alpha-4 0.014 0.036 0.002 0.024 0.333 0.400 IPI00223446.5
Lama5 Laminin subunit alpha-5 0.329 0.643 0.693 0.321 0.917 0.200 IPI00116913.3
Lamb1 Laminin subunit beta-1 0.010 0.012 0.002 0.060 0.012 0.005 IPI00338785.3
Lamb2 Laminin subunit beta-2 precursor 0.029 0.393 0.004 0.321 0.119 0.114 IPI00109612.2
Lamb3 Laminin subunit beta-3 0.243 0.214 0.006 0.060 0.071 0.048 IPI00117093.1
Lamc1 Laminin subunit gamma-1 0.143 0.893 0.045 0.333 0.071 0.310 IPI00400016.1
Lamc2 Laminin subunit gamma-2 0.005 0.012 0.004 0.155 0.488 0.857 IPI00117115.3
Myh10 Myosin, heavy polypeptide 10, non-muscle 0.038 0.107 0.002 0.048 0.012 0.005 IPI00338604.5
Myh11_1 Isoform 1 of Myosin-11 0.357 0.571 0.002 0.298 0.012 0.014 IPI00114894.1
Myh14 Isoform 1 of Myosin-14 0.567 0.060 0.004 0.167 0.012 0.005 IPI00453996.1
Myh6 Myosin-6 0.162 0.048 0.095 0.095 0.012 0.005 IPI00129404.1
Myh9 Myosin-9 0.562 0.155 0.002 0.012 0.012 0.005 IPI00123181.4
Nid1 Nidogen-1 0.019 0.167 0.022 1.000 0.345 0.467 IPI00111793.1
Intracellular cytoplasmic
Aco2 Aconitate hydratase, mitochondrial 0.033 0.333 1.000 1.000 0.333 0.033 IPI00116074.1
Actb Actin, cytoplasmic 1 0.076 0.155 0.227 0.071 0.024 0.500 IPI00110850.1
Ctnna1 Catenin alpha-1 0.900 0.536 0.455 0.202 0.012 0.400 IPI00112963.1
Des Desmin 0.729 0.012 0.006 0.060 0.583 0.010 IPI00130102.4
Dpysl2 Dihydropyrimidinase-related protein 2 1.000 0.631 0.132 0.512 0.048 0.152 IPI00114375.2
Eef2 Elongation factor 2 0.010 0.940 0.697 0.012 0.750 0.010 IPI00466069.3
Hadha Trifunctional enzyme subunit alpha, mitochondrial 0.010 0.012 1.000 0.012 0.012 0.010 IPI00223092.5
Hadhb Trifunctional enzyme subunit beta, mitochondrial 0.400 1.000 1.000 1.000 1.000 0.400 IPI00115607.3
Hbaa1 Putative uncharacterized protein 1.000 0.440 0.182 1.000 0.012 0.033 IPI00110658.1
Hbbb1 LOC100503164 Beta-globin 0.867 0.393 0.069 0.095 0.036 0.095 IPI00762198.2
Myo1c Isoform 2 of Myosin-Ic 0.019 1.000 0.673 0.750 0.893 0.143 IPI00467172.2
Serpinh1 Serpin H1 1.000 1.000 1.000 0.012 1.000 1.000 IPI00114733.1
Tgm2 Protein-glutamine gamma-glutamyltransferase 2 0.005 0.024 0.141 0.012 0.202 0.010 IPI00126861.3
Ywhaz 14-3-3 protein zeta/delta 0.033 1.000 1.000 1.000 1.000 0.033 IPI00116498.1
Intracellular nuclear
Eef1a2 Elongation factor 1-alpha 2 1.000 0.333 1.000 0.012 1.000 1.000 IPI00119667.1
Hist1h2bf_j_l_n Histone H4 0.005 0.631 0.004 0.607 0.012 0.005 IPI00407339.7
Hist1h2bn_f_j_l Histone Type 1-F/J/L. 0.033 0.893 0.552 0.024 0.798 0.019 IPI00114642.4
Hnrnpk Isoform 2 of Heterogeneous nuclear ribonucleoprotein K 0.033 0.083 0.035 0.083 0.821 0.200 IPI00224575.1
Ptrf Polymerase I and transcript release factor 0.400 0.012 0.015 0.012 0.048 0.048 IPI00117689.1
Membrane
Anxa1 Annexin A1 1.000 0.750 1.000 0.024 0.750 1.000 IPI00230395.5
Anxa2 Annexin A2 1.000 0.083 1.000 0.012 0.083 1.000 IPI00468203.3
Atp5a1 ATP synthase subunit alpha, mitochondrial 0.029 0.714 0.232 0.048 0.250 0.005 IPI00130280.1
Atp5b ATP synthase subunit beta, mitochondrial 0.038 0.071 0.429 0.857 0.143 0.071 IPI00468481.2
Ehd2 Eh domain-containing protein 1 0.490 0.036 0.251 0.488 0.131 0.190 IPI00126083.3
Iqgap1 Ras GTPase-activating-like protein IQGAP1 0.052 0.833 0.009 0.024 0.202 0.714 IPI00467447.3
Npnt Isoform 4 of Nephronectin 1.000 0.083 1.000 0.012 0.083 1.000 IPI00124689.1
Samhd1 Mimecan 0.033 1.000 1.000 0.333 1.000 0.033 IPI00120848.1
Slc25a4 ADP/ATP translocase 1 1.000 0.333 0.455 0.012 0.643 0.467 IPI00115564.5
Vdac2 Voltage-dependent anion-selective channel protein 2 0.033 0.333 1.000 0.333 0.333 0.033 IPI00122547.1
Tln1 Talin-1 0.010 0.619 0.002 0.345 0.012 0.129 IPI00465786.3

Values represent p-values from two group comparisons of raw unique peptide hits using the non-parametric test (pre-planned, selected, pairwise comparisons are shown in the top two rows). Protein group assignments were generated from UniProt entries of positively identified proteins (proteins containing two or more unique peptide hits). Grayed boxes with italicized text indicate the treatment group (labeled in the second row) reached statistical significance (p-values less than 0.05) with higher amounts of unique peptide hits than the control group. White boxes with black bolded text indicate the control group (labeled in the top row) contained significantly more unique peptide hits.

Italics – treatment group significantly higher.

Black – control significantly higher (designated above).

Key: Elas = Elastase; Bleo = Bleomycin; Old Elast = Old Elastase; Oldlg = Old Mouse; RLL = Young Mouse, Right Lower Lobe.