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. Author manuscript; available in PMC: 2014 Oct 31.
Published in final edited form as: Osteoporos Int. 2010 Jul 1;22(5):1633–1639. doi: 10.1007/s00198-010-1325-z

Fig. 3.

Fig. 3

Diurnal analysis of osteoblast-associated mRNA expression. The expression profile of mRNA encoding the osteoblast-associated genes in the a femur and b lumbar vertebra of sham-operated (square) and OVX (diamond) mice was determined by RT-PCR using total RNA pooled from n=3 mice per time point (ZT h). The mRNA levels of Alpl, Ibsp, OPG, RANKL, and Runx2 were normalized at the individual time points relative to the housekeeping controls 18S RNA, β-actin, β2-microglobulin, and cyclophilin B. Values displayed are the mean±SD