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Effect of STAT1 and STAT2 gene silencing with siRNA in STAT3-dependent cell lines. DU-145 and PANC-1 cells were treated with 500 nM pooled siRNA for 72 hr, at which time cells were harvested and stained with FITC-annexin V/PI and analyzed by flow cytometry. More than 95% of the cells were observed to be viable following siRNA transfection. Transfection efficiencies ranged from 95 to 98% (not shown).
