Table 1.
Effect of siRNA-mediation inhibition of STATs 1, 2, and 3 on cell survival. Pooled siRNAs were purchased from Santa Cruz Laboratories. They were transfected into cells using Lipofectamine 2000 according to Invitrogen's protocol. Twenty-four hr after transfection, cells were harvested, washed, stained with FITC-annexin V/PI, and analyzed by flow cytometry. Data from a representative experiment are given.
| Cell Line | siRNA | nM | %Apoptotic |
|---|---|---|---|
| DU-145 | None | - | 0 |
| STAT1 | 250 | 0.04 | |
| 500 | 0.01 | ||
| STAT2 | 250 | 0.02 | |
| 500 | 0.07 | ||
| STAT3 | 250 | 50.62 | |
| 500 | 35 | ||
| Control | 250 | 0.02 | |
| 500 | 0.13 | ||
| PANC-1 | None | - | 0 |
| STAT1 | 250 | 0 | |
| 500 | 0.02 | ||
| STAT2 | 250 | 0.01 | |
| 500 | 0.03 | ||
| STAT3 | 250 | 12.1 | |
| 500 | 46.8 | ||
| Control | 250 | 0.07 | |
| 500 | 0 |