Table 2.

Lack of synergy between STAT1 or STAT2 and STAT3 siRNAs. Pooled siRNAs were purchased from Santa Cruz Laboratories. They were co-transfected into cells using Lipofectamine 2000 according to Invitrogen's protocol. Twenty-four hr after transfection, cells were harvested, washed, stained with FITC-annexin V/PI, and analyzed by flow cytometry. % apoptotic data were normalized to 100% transfection efficiency. P value was determined by repeated ANOVA.

Cell Line	siRNA	nM (each siRNA)	% Apoptotic	±SEM	P
DU-145	None	-	0	0	0.97
	STAT3	250	52.8	5.4
	STAT1+STAT3	250	49.8	10
	STAT2+STAT3	250	52.2	9.4
	Control+STAT3	250	50.8	10.7
PANC-1	None	-	0	0	0.47
	STAT3	250	38.2	10.7
	STAT1+STAT3	250	40.2	9.9
	STAT2+STAT3	250	32.7	12
	Control+STAT3	250	37.2	9.5