Table 3.

Effect of anti-STAT3 oligonucleotides on STAT3-null cells. BPH-1 cells and BPH-DN-STAT3 cells were transfected with olignucleotides as described in Materials and Methods. Forty-eight hrs later, cells were harvested and stained with annexin-V and PI to assess apoptosis by flow cytometry. The means plus standard deviations of three experiments are given.

Cell Line	OLG	nM	%Apoptotic	± SD
BPH-1	None	0	0.17	0.27
	13410	100	0	0
		300	0	0
		1000	0.04	0.07
	13410a	100	0	0
		300	0.02	0.01
		1000	0.07	0.1
	13778	100	0	0
		300	0.3	0.29
		1000	0.08	0.14
	13778a	100	0.04	0.03
		300	0.06	0.05
		1000	0.37	0.66
BPH-DN-STAT3	None	0	1.94	1.77
	13410	100	0.4	0.37
		300	0.52	0.46
		1000	0.87	0.12
	13410a	100	0.63	0.55
		300	1.02	0.89
		1000	2.25	1.05
	13778	100	1.29	1.13
		300	0.53	0.46
		1000	1.48	0.16
	13778a	100	0.47	0.41
		300	0.56	0.49
		1000	2.60	2.55