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. 2014 Oct 28;3:131. Originally published 2014 Jun 19. [Version 2] doi: 10.12688/f1000research.4219.2

Figure 7. Enhanced CREB phosphorylation and neurite length in neurons treated with apoptotic MNC-secretomes.

Figure 7.

( a) Cell extracts were prepared after stimulation with hMNC apo sec or cell culture medium as control. Western blot analysis for phospho-CREB revealed a dose dependent activation of CREB in astrocytes and in neurons. ( b) Neuron cultures treated with hMNC apo sec or control medium for five days were stained with methylene-blue. One representative picture of ten is shown. Bar=10 µm ( c) Lengths of neurons treated with hMNC apo sec or cell culture medium as control were calculated using ImageJ software. Bars represent the mean of five different cultures.