Figure 5. Osmotically evoked hypertrophy is prevented by inhibitors of PLC or PKC and hypertrophy may be activated by a Ca²⁺ ionophore or by exposure to high K⁺ saline.

A, exposure (indicated by grey bar) to hypertonic (325 mosmol kg⁻¹) saline causes MNC shrinkage, but not hypertrophy, in the presence of the PLC inhibitor U73122 (1 μm) or the PKC inhibitor bisindolylmaleimide I (1 μm). Exposure to the PKC activator phorbol 12-myristate 13-acetate (0.1 μm; green symbols and line) induced hypertrophy in the absence of an increase in osmolality. B, exposure (indicated by grey bar) to either high K⁺ saline (25 mm) or to the Ca²⁺ ionophore A23187 (10 μm) causes MNCs to hypertrophy. The hypertrophic effect of high K⁺ saline is blocked by the PKC inhibitor bisindolylmaleimide I (1 μm).