Figure 5. MORs on POMC neurons are antagonized by nor-BNI at sufficiently high concentrations.

A, a sample trace demonstrating the reversal of an outward current induced by Dyn A (5 μm) by nor-BNI (1 μm). B, the same concentration of nor-BNI (1 μm) is also sufficient to reverse the outward current induced by higher concentration of DAMGO (10 μm). D and E, compiled data showing the reversal of outward currents induced by Dyn A and DAMGO by nor-BNI (1 μm). C, dose-dependent inhibition of an outward current induced by a near EC₅₀ concentration of DAMGO (500 nm) by 100 nm and 500 nm nor-BNI. F, compiled data demonstrating the reversal of the outward current induced by DAMGO (500 nm) by 100 nm and 500 nm nor-BNI. The amplitude of the outward current induced by DAMGO (500 nm) in the presence of nor-BNI (500 nm) is significantly different than that of DAMGO (500 nm) alone using Tukey's post hoc test (a). P values are represented by asterisks: *P < 0.05, **P < 0.01.