Figure 4. Prior activation of D1Rs attenuated mGluR2/3-induced synaptic depression through activation of a presynaptic AC pathway.
A, prior application of 50 μm SKF38393 for 10 min significantly suppressed the LY-SD (n = 5). B, bar chart summarizing the effect of AC inhibitors and PLC inhibitors on SKF38393-induced suppression of LY-SD. Pre-incubation with the membrane-permeable AC inhibitor MDL-12230A (30 μm, n = 5) blocked the attenuation of LY-SD induced by SKF38393. Bath application of the membrane-permeable PLC inhibitor U73122 (10 μm, n = 4), or intracellular application of the impermeable PLC inhibitor neomycin (0.5 mm, n = 4) failed to block D1R agonist effect. ***P < 0.001, **P < 0.01. Error bars indicate SEM. Upper traces in A are representative EPSPs evoked at time points 1 and 2 for each treatment group.