Figure 2. Modulation of hECN GABA_ARs by diazepam, Zn²⁺ and gaboxadol.

A, left panel: representative whole-cell recording depicting the co-application of diazepam (30 nm and 3 μm, as indicated by bars) to control GABA-evoked responses. A, right panel: modulation of GABA_AR-mediated currents by diazepam (30 nm and 3 μm, n = 17, N = 3). Data are presented as mean percentage modulation with respect to control recordings. No difference was observed between percentage modulation and the batch from which cells were prepared. Calibration bar 50 pA, 50 s. B, left panel: example whole-cell recording depicting the co-application of Zn²⁺ (10 μm and 300 μm, as indicated by bars) to control GABA-evoked responses. B, right panel: mean percentage modulation of control GABA_AR-mediated currents by Zn²⁺ (n = 9, N = 1). Calibration bar 100 pA, 50 s. C, left panel: example whole-cell recording of GABA (3 mm)-evoked currents and gaboxadol (3 μm and 300 μm)-induced currents. C, right panel: mean percentage gaboxadol-induced activation of GABA_AR currents with respect to (w.r.t.) maximum GABA-evoked currents (n = 6–7, N = 1). Calibration bar 500 pA, 50 s.