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. 2014 Oct 31;5:577. doi: 10.3389/fmicb.2014.00577

Figure 4.

Figure 4

Requirement for hydrogen utilization during growth on formate/acetate/sulfate medium. (A,B) Growth of mutants in the periplasmic [NiFeSe] hydrogenase (hysAB), a periplasmic formate dehydrogenase (fdhAB, Dde_0717:Dde_0718), formate:hydrogen lyase (fhl), or of the parent strain. Growth was measured for 2–3 different mutants in each complex, and each point shows the average across three cultures for a strain. (A) Growth in 50 mM formate, 10 mM acetate, and 30 mM sulfate. (B) Growth in 60 mM lactate and 30 mM sulfate. (C,D) Comparisons of gene fitness with formate, lactate, or hydrogen as electron donor. “Fdhs” includes periplasmic formate dehydrogenases and formate:hydrogen lyase. “Moe” includes molybdopterin synthesis genes (Dde_0709, Dde_1390, Dde_0249, Dde_2352, Dde_0230, Dde_3228). “Hys” includes hysAB and maturation genes (Dde_2136, Dde_0364, Dde_0363, Dde_0555). The fitness data is the average of two independent experiments for each of two pools of mutants. Both growth and fitness experiments were performed in MO media with 1 mM sulfide (as reductant) and no added yeast extract or vitamins.