Figure 7.

Cellular degeneration in cerebellum following diabetes. Histological cresyl violet (CV) staining depicted cellular degeneration in cerebellum in terms of lightly stained condensed nissl substance, loss of nucleus and reduced number of Purkinje cells (B–G; red arrows) following STZ-induced diabetes upto the 12 week. The degenerated purkinje cells were clearly demarcated in all the diabetic time points (red arrows). Additionally, cells in molecular and granule cell layer were reduced in number with small lightly stained cell bodies presenting chromatolysis following diabetes (B–G; black arrows) in comparison to the controls having darkly stained large sized cells (A; black arrowhead). In controls, Purkinje cells were darkly stained arranged in a uniform monolayer with centric nuclei (A; red arrowheads). Scale bar = 50 μm.