Figure 8.

Histoarchitectural alterations in cerebellum during diabetes. Hematoxylin and eosin stained diabetic cerebellum presented a marked degeneration and cell death consistently upto the 12th week. Control cerebellar tissue presented intact cerebellar layers having normal Purkinje cells with dendrites and centrally placed nuclei (A; red thin arrow) and resting astroglia (A; blue thin arrow) and microglia (A; green thin arrow). Effect of diabetes was clearly evident in cerebellar cells presenting pathological signs of degeneration and cell death (B–G). 2nd week onwards Purkinje cells were presenting chromatolysis, necrosis/apoptosis (B–G; red and orange thick arrows), pyknosis (B–G; red thick arrows), vacuolation (G; dotted green circle) and neuronophagia (E,F; dotted yellow circle). Astroglia (B–G; thick blue arrow) and microglia (B–G; thick green arrow) activation was also observed in cerebellar layers at all the diabetic time points. Scale bar = 50 μm.