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. Author manuscript; available in PMC: 2014 Oct 31.
Published in final edited form as: Am J Transplant. 2010 Feb 1;10(3):518–526. doi: 10.1111/j.1600-6143.2009.02967.x

Figure 4. Expression of either MHC class I or II, as well as CD80/86, is required on host dendritic cells to promote CD8 T-cell tolerance.

Figure 4

Radiation chimeras prepared as described in Figure 2 received conditioning with Day-1 3Gy TBI and Day 0 anti-CD154 and B10.A BMT in addition to DT treatment. Some animals in each experimental group received anti-CD8 mAb to deplete host CD8 T cells. The percentage of donor chimerism in the Mac-1+ lineage of the peripheral blood at 4 weeks post-BMT for all mice in four separate experiments is shown. Recipient groups included: WT DC (B6 + DTR → B6) n = 22, MHC class I KO DC (KbDb−/− + DTR → B6) n = 9, MHC class II KO DC (CIITA−/− + DTR → B6) n = 15, MHC class I & II KO DC (KbDb−/−CIITA−/− + DTR → B6) n = 6, MHC class I and II KO DC + anti-CD8 n = 4, CD80/86 KO DC (CD80/86−/− + DTR → B6) n = 7 and No DC (DTR → B6) n = 11. The dotted line at 5% donor chimerism depicts our cutoff for referring to an animal as a chimera.