FIG. 2.

The B. burgdorferi Sh-2-82 chromosome contains strain B31 linear plasmid lp21-like sequences. Whole-genomic Sh-2-82 DNA was prepared in agarose blocks and subjected to CHEF electrophoresis, and Southern analysis was performed with probe 2 (Table 1 and Fig. 1) as described in Materials and Methods. Culture passage number is indicated above the lanes; “std” is HindIII cut plus a ladder of whole bacteriophage λ DNA.