FIG. 4.

Linear plasmid lp21-like sequences lie near the right end of some B. burgdorferi chromosomes. Bacterial DNA was prepared, restricted, separated by CHEF electrophoresis, and subjected to Southern analysis with probe 5 (Table 1 and Fig. 1) DNA from the right unique region of plasmid lp21. Strain names and restriction enzyme cleavage are indicated above the lanes (the p7, p166, and p320 lanes contain Sh-2-82 DNA from cultures with those passage numbers); “std” is HindIII cut plus a ladder of whole bacteriophage λ DNA.