Figure 4.

ncOGT activity is linearly dependent on the concentration of Nup62. Increasing amounts of the 6 × His-tagged Nup62 substrate were used as indicated. ncOGT containing lysates were used for these experiments. Glycosyltransferase activity was detected using Staudinger ligation with Biotin-Phosphine reagent. Experiments were performed in duplicate and quantified using the Odyssey Infrared Imaging System at 800 nm. Values were normalized such that no substrate was set to 0. Error bars represent range.