Figure 5.

Inhibition of ncOGT activity by compounds 4 and 5. Increasing amounts of inhibitors 4 (A) and 5 (B) were added to the Ni-NTA Plate OGT Assay (as indicated). Glycosyltransferase activity was detected using Staudinger ligation with Biotin-Phosphine reagent. Experiments were performed in triplicate (n = 3) and quantified using the Odyssey Infrared Imagining System at 800 nm. IC₅₀ values were calculated using SigmaPlot 12.5 software employing “Four parameter logistic equation in the standard curve analysis”. Error bars represent standard deviations.