Interactive multigroup cloud plot with
customized metabolomic data
visualization. Metabolite features whose level varies significantly
(p < 0.01) across wild-type and different mutants
are projected on the cloud plot depending on their retention time
(x-axis) and m/z (y-axis). Each metabolite feature is represented
by a bubble. Statistical significance (p-value) is represented by
the bubble’s color intensity. The size of the bubble denotes
feature intensity. When the user scrolls the mouse over a bubble,
feature assignments are displayed in a pop-up window (m/z, RT, p-value, fold change).
When a bubble is selected by a “mouse click”, the EIC,
Box-Whisker plot, Posthoc, and METLIN hits appear on the main panel.
Each bubble is linked to the METLIN database to provide putative identifications
based on accurate m/z. The variation
pattern of glutamic acid (m/z 146.0468,
MS/MS METLIN match) across different mutants is shown by a box–whisker
plot.