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. 2014 Oct 31;9(10):e110375. doi: 10.1371/journal.pone.0110375

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© 2014 Montgomery et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A, B) Mef2>RFP pupae reared on indicated concentration of MeHg to stage P6 (A) or P12 (B) and imaged by bright light and red fluorescence to reveal DLM morphology. (C) Close-up image of selected panel from A and B. The solid arrow indicates reduced DLM bundle size and defects in DLM bundle splitting with MeHg. Open arrows indicate displacement of attachment sites of DVM bundles. Asterisks (*) indicates failure of extension and anchoring of the DLM resulting in myofibers coalesced in a ball. The development of eyes and bristles appear unaffected by MeHg treatment.