Table 1. Top Lanthipeptides Discovered by the RiPPquest Automated Peptidogenomics Pipelinea.
| peptide | species | MS/MS type | MS/MS precursor [m/z] | z | p-value | annotation |
|---|---|---|---|---|---|---|
| informati-peptin | S. viridochromogenes DSM 40 736 | CID | 1065.56 | 2 | 9.2 × 10–8 | TDGGGASTVSLLSCISAASVLLCL (6 Dehyd) |
| S. viridochromogenes DSM 40 736 | HCD | 1065.54 | 2 | 0.005 | TDGGGASTVSLLSCISAASVLLCL (6 Dehyd) | |
| S. viridochromogenes DSM 40 736 | CID | 1015.02 | 2 | 0.013 | DGGGASTVSLLSCISAASVLLCL (6 Dehyd) | |
| S. viridochromogenes DSM 40 736 | HCD | 1015.02 | 2 | 0.03 | DGGGASTVSLLSCISAASVLLCL (6 Dehyd) | |
| S. viridochromogenes DSM 40 736 | CID | 957.51 | 2 | 0.0026 | GGGASTVSLLSCISAASVLLCL (6 Dehyd) | |
| S. viridochromogenes DSM 40 736 | HCD | 957.50 | 2 | 0.0098 | GGGASTVSLLSCISAASVLLCL (6 Dehyd) | |
| S. viridochromogenes DSM 40 736 | CID | 928.49 | 2 | 0.0003 | GGASTVSLLSCISAASVLLCL (6 Dehyd) | |
| S. viridochromogenes DSM 40 736 | HCD | 928.99 | 2 | 0.012 | GGASTVSLLSCISAASVLLCL (6 Dehyd) | |
| AmfS | S. griseus IFO 13 350 | CID | 1028.04 | 2 | 2.5 × 10–10 | GSQVSLLVCEYSSLSVVLCTP (4 Dehyd) |
| SRO-2212 | S. roseosporus NRRL 15 998 | CID | 1107.07 | 2 | 6.3 × 10–10 | TGSQVSLLVCEYSSLSVVLCTP (4 Dehyd) |
| S. roseosporus NRRL 15 998 | HCD | 1107.06 | 2 | 5.3 × 10–7 | TGSQVSLLVCEYSSLSVVLCTP (4 Dehyd) | |
| S. roseosporus NRRL 15 998 | HCD | 738.37 | 3 | 6.9 × 10–5 | TGSQVSLLVCEYSSLSVVLCTP (4 Dehyd) | |
| S. roseosporus NRRL 15 998 | CID | 1056.55 | 2 | 2.0 × 10–9 | GSQVSLLVCEYSSLSVVLCTP (4 Dehyd) | |
| S. roseosporus NRRL 15 998 | HCD | 1065.53 | 2 | 0.001 | GSQVSLLVCEYSSLSVVLCTP (3 Dehyd) | |
| S. roseosporus NRRL 15 998 | HCD | 710.693 | 3 | 0.001 | GSQVSLLVCEYSSLSVVLCTP (3 Dehyd) | |
| S. roseosporus NRRL 15 998 | CID | 1142.58 | 2 | 3.8 × 10–6 | ATGSQVSLLVCEYSSLSVVLCTP (4 Dehyd) | |
| S. roseosporus NRRL 15 998 | HCD | 1142.57 | 2 | 0.013 | ATGSQVSLLVCEYSSLSVVLCTP (4 Dehyd) | |
| S. roseosporus NRRL 15 998 | HCD | 1028.02 | 2 | 5.0 × 10–5 | SQVSLLVCEYSSLSVVLCTP (4 Dehyd) | |
| SRO-3108 | S. roseosporus NRRL 15 998 | CID | 1555.68 | 2 | 1.2 × 10–6 | TTWACATVTLTVTVCSPTGTLCGSCSMGTRGCC (9 Dehyd) |
| S. roseosporus NRRL 15 998 | HCD | 1564.17 | 2 | 0.02 | TTWACATVTLTVTVCSPTGTLCGSCSMGTRGCC (9 Dehyd) | |
| S. roseosporus NRRL 15 998 | CID | 1582.81 | 2 | 1.0 × 10–7 | {TTWAC}+54ATVTLTVTVCSPTGTLCGSCSMGTRGCC (9 Dehyd) | |
| S. roseosporus NRRL 15 998 | CID | 1636.71 | 2 | 0.001 | {TTWAC}+162ATVTLTVTVCSPTGTLCGSCSMGTRGCC (9 Dehyd) | |
| S. roseosporus NRRL 15 998 | CID | 1037.11 | 2 | 0.0001 | TTWACATVTLTVTVCSPTGTLCGSCSMGTRGCC (9 Dehyd) | |
| S. roseosporus NRRL 15 998 | HCD | 1037.11 | 3 | 0.0007 | TTWACATVTLTVTVCSPTGTLCGSCSMGTRGCC (9 Dehyd) | |
| S. roseosporus NRRL 15 998 | CID | 1055.59 | 3 | 0.001 | {TTWAC}+54ATVTLTVTVCSPTGTLCGSCSMGTRGCC (9 Dehyd) | |
| S. roseosporus NRRL 15 998 | CID | 1067.92 | 3 | 0.0001 | TVTVCSPTGTLCGSCSMGTRGCC (5 Dehyd) | |
| S. roseosporus NRRL 15 998 | HCD | 1067.42 | 2 | 7.7 × 10–7 | TVTVCSPTGTLCGSCSMGTRGCC (5 Dehyd) | |
| S. roseosporus NRRL 15 998 | CID | 1123.99 | 2 | 4.6 × 10–7 | LTVTVCSPTGTLCGSCSMGTRGCC (5 Dehyd) | |
| S. roseosporus NRRL 15 998 | CID | 1166.47 | 2 | 0.0002 | TLTVTVCSPTGTLCGSCSMGTRGCC (6 Dehyd) | |
| S. roseosporus NRRL 15 998 | CID | 1215.54 | 2 | 0.0003 | VTLTVTVCSPTGTLCGSCSMGTRGCC (6 Dehyd) | |
| S. roseosporus NRRL 15 998 | CID | 1256.13 | 2 | 0.038 | TVTLTVTVCSPTGTLCGSCSMGTRGCC (7 Dehyd) | |
| S. roseosporus NRRL 15 998 | CID | 1075.42 | 2 | 0.009 | TVTVCS{PTGTLCGSCSMG}+16TRGCC (4 Dehyd) | |
| S. roseosporus NRRL 15 998 | CID | 1174.49 | 2 | 5.6 × 10–8 | TLTVTVCSPTGTLCGSCSMGTRGCC (5 Dehyd) | |
| SapB | S. coelicolor A3(2) | CID | 1013.99 | 1 | 0.02 | TGSRASLLLCGDSSLSITTCN (4 Dehyd) |
| SapB | S. lividans TK24 | HCD | 934.96 | 2 | 0.016 | SRASLLLCGDSSLSITTCN (4 Dehyd) |
| S. lividans TK24 | HCD | 1013.99 | 2 | 4.0 × 10–5 | TGSRASLLLCGDSSLSITTCN (4 Dehyd) | |
| SAL-2242 | S. albus J1074 | HCD | 1062.53 | 2 | 5.7 × 10–5 | GSQISLLICEYSSLSVTLCTP (4 Dehyd) |
| S. albus J1074 | HCD | 1113.06 | 2 | 0.015 | TGSQISLLICEYSSLSVTLCTP (5 Dehyd) | |
| S. albus J1074 | HCD | 1122.06 | 2 | 0.017 | TGSQISLLICEYSSLSVTLCTP (4 Dehyd) |
a
Number of dehydrations involved in lanthipeptide processing are shown in each case. Abbreviations: m/z, mass-to-charge ratio; z, precursor ion charge; dehydr, dehydration. Corresponding annotated spectra are shown in SI Figure S4.