FIG. 3.

Detection of Psp response-inducing proteins. Strains containing plasmid pVLT35 (vector) or derivatives encoding the indicated genes were grown as described in the Fig. 2 legend. Total cell proteins were separated by SDS-12.5% PAGE, transferred to nitrocellulose, and detected with a penta-His antibody as described in Materials and Methods. The signals generated by the six-His-tagged proteins are underscored with asterisks. Molecular weights of the detected proteins were estimated by comparison to a prestained molecular weight marker (not shown). Note that the YPO0432 ortholog did not resolve from the dye front on the gel owing to its small size (approximately 6 kDa). −, no IPTG; +, 0.2 mM IPTG.