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. 2014 Oct 31;9(10):e109916. doi: 10.1371/journal.pone.0109916

Figure 3. Assaying glucose oxidation.

Figure 3

A. Schematic illustration of biochemical pathway for glucose oxidation. The NADH produced in the cytosol as glucose is converted to pyruvate is imported into the mitochondria via the malate-aspartate shuttle and regenerated via the ETC to maintain continuous glucose oxidation. B. Kinetic OCR response of PC-3 cells to glucose (10 mM); C. OCR response to glucose (10 mM), oligomycin (1 µM) and FCCP (0.3 µM). PC-3 cells were plated at 25,000/well in XF24 V7 culture plates. The assay medium was the substrate-free base medium. The OCR values were not normalized. A representative experiment out of three is shown here. Each data point represents mean ± SD, n = 4.