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. 2014 Oct 31;9(10):e109916. doi: 10.1371/journal.pone.0109916

Figure 4. Assaying glutamine oxidation and demonstrating transaminase pathway activity.

Figure 4

A. Schematic illustration of biochemical pathway for glutamine oxidation in the mitochondria. B. Kinetic OCR response in SF188f cells to glutamine (4 mM). SF188f cells were plated at 20,000 cells/well in XF24 cell culture plates 24–28 hours prior to the assays. The assay medium was the substrate-free base medium. The OCR value was not normalized. A representative experiment out of three is shown here. Each data point represents mean ± SD, n = 4. C. OCR response (% of baseline) in SF188f cells to glutamine (4 mM) and AOA (100 µM). Glutamine-induced OCR reached 60% over the baseline (OCR at measurement 6 divided by that at measurement 3) while AOA addition reduced it to 20% (OCR at Measurement 9 divided by measurement 3). SF188f cells were plated at 20,000 cells/well in XF24 V7 cell culture plates 24–28 hours before the assays. The % OCR was plotted using measurement 3 as the baseline. The assay medium was the substrate-free base medium. A representative experiment out of three is shown here. Each data point represents mean ± SD, n = 4.