Table 1. Immunohistological and molecular analyses.
Patients | Lesion | HPV (ISH) | HPV (InnoLiPa) | HPV (consensus primers) | MCPyV | HPyV6 | HPyV7 | BRAF | NRAS | HRAS | KRAS | Pi3KCA |
1 | SCC | - | - | - | - | - | - | - | - | - | G12C | - |
1 | SCC | - | - | - | - | - | - | - | - | G13D/H27H | G12C | E542K |
2 | SCC | - | - | - | - | - | - | - | - | G12D | G12C | - |
2 | SCC | - | - | - | - | - | - | - | - | Q61L | G12C | - |
2 | VP | - | - | - | - | - | - | - | - | - | G12C | - |
2 | VP1 | - | - | - | - | - | - | - | - | - | G12C | - |
3 | VP | - | - | - | - | - | - | - | - | Q61L | G12C | E545X |
4 | VP | - | - | - | - | - | - | - | - | - | G12C | - |
5 | VP | - | - | - | - | - | - | - | - | H27H | NI | - |
5 | VP | - | +3 | - | - | - | - | - | - | H27H/Q61L | NI | - |
5 | VP2 | - | - | - | - | - | - | - | - | H27H/F82L | - | - |
6 | VP | - | - | - | - | - | - | - | - | H27H/Q61L | G12C | - |
6 | SCC | - | - | - | - | - | - | - | - | H27H/Q21L | NI | E542K |
7 | VP | - | - | - | - | - | - | - | - | - | NI | - |
8 | VP2 | - | - | - | - | - | - | - | - | G12D/K16K/H27H | G12C | - |
8 | VP | - | - | - | - | - | - | - | - | H27H | NI | - |
8 | KA | - | - | - | - | - | - | - | - | G12D/H27H | NI | - |
8 | SCC | - | - | - | - | - | - | - | - | H27H | G12C | - |
9 | VP | - | - | - | - | - | + | - | - | G12N | - | E542K |
10 | VP | - | - | - | - | - | - | - | - | Q61L | - | - |
10 | VP | - | HPV39 | - | - | - | - | - | - | G12R | - | NI |
10 | VP | - | - | - | + | - | - | - | - | - | - | - |
10 | SCC | - | - | - | - | - | - | - | - | G12V | - | - |
11 | VP | - | - | - | - | - | - | - | - | H27H | - | - |
11 | VP | - | - | - | - | - | - | - | - | H27H | - | - |
11 | VP | - | - | - | - | - | - | - | - | H27H/Q61L | - | - |
12 | VP | - | - | - | + | - | - | - | - | H27H | G12D | - |
One VP had a histological appearance of possible regressive keratoacanthoma,
while 2 VPs displayed slightly invasion.
For one sample, HPV was detected by InnoLipa and could not be further characterized. -: no mutation or HPV, MCPyV, HPyV6 or HPyV7 was detected within the sample. NI (not informative): for 7 tumors, no results were obtained for KRAS or Pi3KCA sequencing.