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. 2014 Oct 31;9(10):e110478. doi: 10.1371/journal.pone.0110478

Table 1. Immunohistological and molecular analyses.

Patients Lesion HPV (ISH) HPV (InnoLiPa) HPV (consensus primers) MCPyV HPyV6 HPyV7 BRAF NRAS HRAS KRAS Pi3KCA
1 SCC - - - - - - - - - G12C -
1 SCC - - - - - - - - G13D/H27H G12C E542K
2 SCC - - - - - - - - G12D G12C -
2 SCC - - - - - - - - Q61L G12C -
2 VP - - - - - - - - - G12C -
2 VP1 - - - - - - - - - G12C -
3 VP - - - - - - - - Q61L G12C E545X
4 VP - - - - - - - - - G12C -
5 VP - - - - - - - - H27H NI -
5 VP - +3 - - - - - - H27H/Q61L NI -
5 VP2 - - - - - - - - H27H/F82L - -
6 VP - - - - - - - - H27H/Q61L G12C -
6 SCC - - - - - - - - H27H/Q21L NI E542K
7 VP - - - - - - - - - NI -
8 VP2 - - - - - - - - G12D/K16K/H27H G12C -
8 VP - - - - - - - - H27H NI -
8 KA - - - - - - - - G12D/H27H NI -
8 SCC - - - - - - - - H27H G12C -
9 VP - - - - - + - - G12N - E542K
10 VP - - - - - - - - Q61L - -
10 VP - HPV39 - - - - - - G12R - NI
10 VP - - - + - - - - - - -
10 SCC - - - - - - - - G12V - -
11 VP - - - - - - - - H27H - -
11 VP - - - - - - - - H27H - -
11 VP - - - - - - - - H27H/Q61L - -
12 VP - - - + - - - - H27H G12D -
1

One VP had a histological appearance of possible regressive keratoacanthoma,

2

while 2 VPs displayed slightly invasion.

3

For one sample, HPV was detected by InnoLipa and could not be further characterized. -: no mutation or HPV, MCPyV, HPyV6 or HPyV7 was detected within the sample. NI (not informative): for 7 tumors, no results were obtained for KRAS or Pi3KCA sequencing.