Figure 1.

Experimental setups. (A) For the GAME configuration, the protein is mixed with the PEG hydrogel and polymerized prior to the introduction of the capillary in the agarose gel. (B) For the 3L configuration, the protein–agarose or the protein–PEG (prepolymeric) solution is first loaded (first layer). For the latter, the protein–PEG solution was subjected to UV treatment to induce the polymerization. This was followed by the gentle addition of the prepolymeric solution of PEG (20, 35, or 50% w/w concentrations), which was also polymerized by UV exposition (second layer or plug). The last step in both experimental setups is the addition of the crystallization solution (third layer).