Table 1. X-ray Data Collection Statistics for Lysozyme Crystals Grown in 10% (w/w) PEG Hydrogel and in 0.2% (w/v) Agarose Gel by the 3L Counterdiffusion Method (Numbers in Parentheses Indicate the Statistics for the High-Resolution Shell).
| PEG hydrogel | agarose gel | |||||
|---|---|---|---|---|---|---|
| crystal size (mm3) | 0.21 × 0.0.202 | 0.22 × 0.152 | 0.21 × 0.202 | 0.48 × 0.252 | 0.38 × 0.222 | 0.54 × 0.182 |
| space group | P43212 | P43212 | P43212 | P43212 | P43212 | P43212 |
| cell dimensions: | ||||||
| a = b, c (Å) | 79.12, 38.07 | 78.71, 37.63 | 79.16, 38.15 | 79.15, 38.10 | 79.15, 38.10 | 79.16, 38.09 |
| resolution (Å) | 25.50–1.85 (1.89–1.85) | 25.50–1.85 (1.89–1.85) | 25.50–1.85 (1.89–1.85) | 25.50–1.85 (1.89–1.85) | 25.50–1.85 (1.89–1.85) | 25.50–1.85 (1.89–1.85) |
| Rsym (%) | 3.67 (42.35) | 4.90 (46.77) | 2.72 (23.13) | 1.96 (13.00) | 4.33 (64.80) | 3.9 (42.8) |
| I/σI | 20.00 (2.44) | 14.94 (2.27) | 25.61 (4.23) | 35.66 (6.89) | 17.49 (1.61) | 18.93 (2.36) |
| completeness (%) | 99.4 (95.0) | 99.5 (94.0) | 99.3 (93.0) | 98.00 (85.5) | 97.4 (92.0) | 98.7 (88.5) |
| unique reflections | 10768 (479) | 10554 (469) | 10784 (463) | 10630 (425) | 10573 (457) | 10707 (583) |
| multiplicity | 13.85 (3.81) | 13.47 (4.00) | 13.89 (3.85) | 13.85 (3.81) | 13.73 (3.83) | 13.81 (4.03) |
| Wilson B factor (Å2) | 12.8 | 12.6 | 13.5 | 13.0 | 12.9 | 12.1 |
| CC1/2 | 0.99 (0.95) | 0.99 (0.54) | 0.99 (0.73) | 0.99 (0.95) | 0.99 (0.75) | 0.99 (0.68) |