. Author manuscript; available in PMC: 2015 Jul 7.

Published in final edited form as: Chembiochem. 2014 Jun 6;15(10):1452–1458. doi: 10.1002/cbic.201402046

Table 2.

Bisubstrate Model Comparison for sp-IDI-2

Mechanism	k_cat (s⁻¹)		K_{i FMN} (μM)		K_IPP (μM)		K_FMN (μM)		Residual sum of squares
Mechanism	Mean	S.E.	Mean	S.E.	Mean	S.E.	Mean	S.E.	Residual sum of squares
Ordered Sequential FMN binding first	0.311	0.062	1.63	0.37	75.4	23.8	-	-	0.004654
Ordered Sequential IPP Binding first	0.127	0.009	−0.941	0.123	−107	12	-	-	0.004279
Random sequential	0.203	0.050	3.12	1.51	34.8	19.1	−0.672	0.265	0.004394

Assay conditions: 60 nM apo-S. pneumoniae type II IDI, 200 mM HEPES, pH 7.0, 2 mM MgCl₂, 2 mM NADPH, 0.14 mg/mL BSA, 23.5 – 85.5 mM IPP and 0.930 – 3.38 mM FMN at 37 °C.