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. Author manuscript; available in PMC: 2014 Oct 31.
Published in final edited form as: Nat Struct Mol Biol. 2010 Feb 14;17(3):379–388. doi: 10.1038/nsmb.1771

Figure 5.

Figure 5

Structure of the [AF]G•A-2 Dpo4 post-extension ternary complex containing two molecules per AU with different positions of the partner A14 base. (a) Schematic of the expected template/primer-dTTP pairing. (b) Schematic of the observed base pairing arrangement within the Dpo4 active site of molecule 1, with the A14 apparently oriented outside the helix. (c) The active site of molecule 1. [AF]G(anti) at the (–2) position opposite primer A14 base. Simulated annealing Fo-Fc omit map contoured at 3σ level is colored in blue and at 2σ level is colored in gray (2.10 Å resolution). (d) The A14 base positioned opposite [AF]G(anti), appears to be oriented outside the helix on the minor groove side. (e) Schematic of the observed base pairing arrangement within the Dpo4 active site of molecule 2 with A14 apparently oriented inside the helix. (f) The active site of molecule 2. Simulated annealing Fo-Fc omit map contoured at 3σ level is colored in blue and at 2σ level it is colored in gray. (g) The A14 base positioned opposite [AF]G(anti), appears to be oriented inside the helix.