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The three VTL genes were over-expressed in the nramp3/nramp4 double mutant background under the control of the 35S promoter and root length was determined after 5 days of growth. Seedlings were grown without added Fe. Seedlings were grown on agarose Petri plates as described in the Materials and Methods. Asterisks indicate significant differences from the wild-type (p<0.001).
