Figure 6. Complementation of the vit1-1 mutant by over-expression of AtVTL1, AtVTL2 or AtVTL5.

The three VTL genes were over-expressed under the control of the 35S promoter in the vit1-1 background. Root length was determined after 13 days of growth on agarose Petri plates as described in the Materials and Methods ion ES medium without added Fe and in the presence of the Fe²⁺ chelator, Ferrozine. Asterisks indicate significant differences from the wild-type (p<0.001). Shown are results from 2 independent transformants.