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Seedlings were grown on agarose petri plates as described in the Materials and Methods. Growth conditions were: ES medium (ES); “0”, ES without Fe or Zn or at the concentrations indicated. RT-qPCR was standardized to the expression of the ACTIN2. Symbols indicate significant differences to expression in the ES medium in three biological replicates: **, p<0.001; *, p<0.01; and +, p<0.05.
