Figure 5. The amount of bond coupling, or additivity of bond energies during cooperative binding steps does not significantly affect assembly yields above a small threshold.

Yield of a 3×3 square grid complex as a function of the bond coupling constant, under many isothermal assembly conditions (solid lines, color) and after an anneal (black) for reaction time . Dashed lines show yields at thermodynamic equilibrium for isothermal conditions with the same color. Error bars <1%.