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. 2004 Jul;186(13):4262–4275. doi: 10.1128/JB.186.13.4262-4275.2004

FIG. 7.

FIG. 7.

PhoP∼P activates transcription from PA4 and PE2 by using different forms of RNAP. (A) Expression of PA3 and PA4 requires EσA. Lane M contains the 200-nucleotide marker. Lanes 1 to 5 and 7 to 13 contain reconstituted EσA. Lane 6 contains core RNAP with no sigma factor added. Lanes 1 and 7 contain no PhoP or PhoP∼P. Lanes 2 to 5 contain increasing amounts of PhoP (0.1 to 5.0 pmol). Lane 6 contains 5.0 pmol of PhoP∼P. Lanes 8 to 13 contain increasing amounts of PhoP∼P (0.1 to 5 pmol). Lanes 8 to 13 contain equal molar amounts of PhoP and *PhoR plus ATP for phosphorylation of PhoP. (B) PhoP∼P specifically activates the PE2 EσE promoter of the phoPR operon. The 100-nucleotide marker is in the lane marked M. Lanes 1 to 8 contain reconstituted EσE plus the phoPR template. Lanes 2 to 4 contain increasing amounts (0.5, 1, and 5 pmol) of PhoP. Lanes 5 to 8 contain increasing amounts (0.25, 0.5, 1, and 5 pmol) of PhoP∼P. Lanes 9 to 13 contain reconstituted EσE plus the spoIIID template. Lanes 10 and 11 contain 1 and 5 pmol of PhoP, respectively. Lanes 12 and 13 contain 1 and 5 pmol of PhoP∼P, respectively.